哥哥高坂京介（17岁）和妹妹高坂桐乃（14岁）兄妹两人的关系近几年处于冷战状态。从某个时间点开始，桐乃看到哥哥时连打招呼都没有，只会用像是看到脏东西般的眼神瞪他。京介认为这种关系也会一直持续下去。某天，京介在门口捡到从来没看过的魔法少女动画《星尘☆小魔女梅露露》的DVD盒子，里面竟然不是动画的DVD，而是成人游戏《和妹妹恋爱吧♪ 》的光碟。京介在晚餐时用梅露露的话题试探谁是光碟的物主，但只得到父母充满偏见的否定意见。就在那天晚上，连打招呼都不会的妹妹突然来到京介房间，并且说她有事情想要商量。原来桐乃迷上了萌系动画以及美少女游戏中的妹系角色，却都不敢告诉别人，只能一直闷在心里。被哥哥知道了自己的秘密后，从此开始了和哥哥之间的“人生咨询”。
我的妹妹哪有这么可爱 (季节 1)
My Little Sister Can't Be My Love (集 1) 7.0
播出日期: 2010-10-03
运行时间: 25 min
概述: For years, ordinary high-schooler Kousaka Kyousuke has had an icy relationship with his younger sister Kirino, a star athlete who is also a star student.
My Little Sister Can't Bring Me to an IRL Meet (集 2) 7.0
播出日期: 2010-10-10
运行时间: 25 min
概述: While being grilled by Kirino over why he hasn't made any progress in the game she ordered him to complete, it occurs to Kyousuke that she needs some friends that she can talk to without concealing her otaku interests.
My Little Sister Can't Be This Cute (集 3) 7.5
播出日期: 2010-10-17
运行时间: 25 min
概述: Kirino finds her first otaku friends at the IRL meet: "Kuroneko" and "Saori Bajeena." They spend their days deep in discussions over their otaku interests. Seeing Kirino this way puts Kyousuke's mind at ease.
My Little Sister Can't Be Going to Summerket (集 4) 7.0
播出日期: 2010-10-24
运行时间: 25 min
概述: One day, Kirino's non-otaku, "public" friends—Aragaki Ayase and Kurusu Nanako—come over to visit. Kyousuke is harshly told by Kirino to not let her friends see him.
My Little Sister's Best Friend Can't Be This XX (集 5) 7.0
播出日期: 2010-10-31
运行时间: 25 min
概述: On the way home from an enjoyable Summerket. They bump into Aragaki Ayase, Kirino's best friend.
My Childhood Friend Can't Be This Cute (集 6) 5.0
播出日期: 2010-11-07
运行时间: 25 min
概述: Kyousuke is asked about his relationship with Manami by his friend, Akagi. Akagi is exasperated when Kyousuke answers that, although they don't have a romantic relationship, he doesn't want her to be taken by anyone else.
My Little Sister Can't Be a Novelist (集 7) 5.0
播出日期: 2010-11-14
运行时间: 25 min
概述: Kyousuke comes home one day and is startled to find Kuroneko loitering in the living room by herself.
My Little Sister Can't Be Animated (集 8) 7.5
播出日期: 2010-11-21
运行时间: 25 min
概述: Kirino has made her debut as an author. Reader response has been overwhelming, and her editor has even better news to report.
My Little Sister Can't Be This Absorbed Into Eroge (集 9) 5.0
播出日期: 2010-11-28
运行时间: 25 min
概述: Kirino has been acting strangely antsy for the past few days. The reason is that the little sister game she ordered hasn't arrived yet.
My Little Sister Can't Be Cosplaying Like This (集 10) 7.5
播出日期: 2010-12-05
运行时间: 25 min
概述: One day, Ayase calls Kyousuke out of the blue and asks him to see her. She wants to get Kirino a present, and wants him to think of what she would be happiest to get.
My Little Sister Can't Be This Maid (集 11) 7.0
播出日期: 2010-12-12
运行时间: 25 min
概述: Sent out to buy groceries by his mother, Kyousuke bumps into Manami at the supermarket. They get to talking, and they eventually arrange for Manami to come visit for the first time in ages.
My Little Sister's Advice Can't End Here (GOOD END) (集 12) 5.0
播出日期: 2010-12-19
运行时间: 25 min
概述: Ever since the events of the previous episode, Kyousuke has been nervous about his improved relationship with his sister.
